Wing Morphology – How Native are my Bees?

ian Queen Rearing and Bee Breeding ,

Wing Morphology – How native are my bees?

In the spring of last year, I decided to investigate how native my bees were, so I conducted wing morphology on all of my colonies, a technique which involves measuring positions of vein intersections on the bee’s main wing.  The results were a surprise, as it looked like my bees are native, or at least near-native anyway.

Using the software tool CBeeWing, colonies showed results which classified the samples as being between 80%-100% Apis mellifera mellifera.

Where did I get my native or near-native bees ?

Six years earlier, I caught a small swarm from my garden fence, here in rural Northumberland.  It would have struggled to fill a pint pot.  I had five colonies at the time, but these bees were different.  They had less colour on their abdomen and they behaved differently.  I put them in a polystyrene nuc.  In the evening they filled the entrance and looked out, so all you could see was a sea of little bee faces starring out of the small round hole.  They seemed considered and organised and I’d swear they were watching my every move.  None of my other bees did this or had this attitude!

The rest of the season they flourished, they were very thrifty on stores during the winter and appeared to get going quickly as they went into the spring.  They were a pleasure to handle and they always appeared to be purposeful and effective in whatever they were doing.

I decided then that I wanted all future queens to come from this colony.

How do I carry out wing morphology ?

The technique I use for wing morphology involves removing the right wing from a sample of around 30 bees from a colony.  These wings are placed carefully on a piece of sticky side up sellotape.  A glass microscope slide is placed over the wings and the sellotape is cut to size and labelled.  This slide is then scanned at the highest resolution on my all-in-one printer to produce a .jpg file.

I tried free software for the analysis of honey bee wings but decided to buy the licence for CBeeWing from Cybis and to download it.  The .jpg file from the scanner is opened in CBeeWing and the key points of the veins on each of the wings is plotted using the system.  It takes some time to learn how to do this but after a couple of samples the process speeds up so you can do a sample of 30 wings in about 15 minutes.

The three main measures that are used for wing morphology are cubital index, discoidal shift and Hantel index.

 

The next stage is to go into the analysis part of the CBeeWing software and to look at the coordinates produced.  The software shows how these coordinates fit with the three main measures used for wing morphology analysis, against the normal ranges of different strains of honey bees.

The results of the analysis are shown in the small windows at the bottom of the screen and can be printed out in a report.

Summary

The wing morphology analysis I carried out last year showed that the samples of bees taken from my colonies, ranged from between 80% to 100% of the profile of Apis mellifera mellifera.

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6 Comments

  1. Nice to know there are still some of these native bees about. Hope you manage to breed more queens to populate your surrounding area. I’m sure neighbouring bee keepers, perhaps new beekeepers, would like colonies like this.

  2. Hi Ian

    Can you let us know the model of scanner you use. I am looking to get one but not sure which type would have sufficient resolution and/or cope with a small slide. Thanks

    1. Sure. The all-in-one printer I have is a few years old, its a HP photosmart C5180. The scanner resolution is upto 4800 x 4800 dpi, which is achievable on most A4 printer/scanners, you really don’t need a special slide scanner. Just set the highest resolution you can on your printer and in the preview of the scan limit the area to the area of the slide.

  3. Ian
    Are you willing to answer individual questions from beekeepers on general management?
    For instance for me, out of 3 hives going into winter one died despite adequate stores
    Of the remaining two I find today that one is queenless with a fair amount of bees, no eggs larvae nor capped brood and unhappy bees
    The third colony has eggs and some capped brood in a super with plenty of stores Queen was found and marked
    I have heard differing opinions as what to do with the queenless hive. Should I combine and increase the flying force or just let die?

    1. What about doing a risk assessment. If they are healthy (no sign of nosema poo, phoetic mite, deformed wings or shaking bees) then you may decide to merge them. If your not sure then don’t take the risk.

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